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CN103045727A 一种与中国荷斯坦奶牛产奶性状及体细胞评分相关的SNP标记及其应用 失效 - 权利终止

一种与中国荷斯坦奶牛产奶性状及体细胞评分相关的SNP标记及其应用
摘要：
本发明涉及一种与中国荷斯坦奶牛产奶性状及体细胞评分相关的SNP标记及其应用。所述SNP标记的位点位于中国荷斯坦奶牛HAL基因如SEQ ID NO.1所示序列的197bp位点处，此处位点的碱基为G或A，此处碱基为G的中国荷斯坦奶牛的乳蛋白含量显著高于此处碱基为A的中国荷斯坦奶牛，而体细胞评分前者显著低于后者。本发明还提供了用于检测所述SNP标记的引物及含有该引物的试剂盒。本发明还提供了所述SNP标记在鉴定乳蛋白量高、体细胞评分低的中国荷斯坦奶牛优势品种中的应用。本发明还提供了所述SNP标记在中国荷斯坦奶牛分子标记辅助育种中的应用。本发明的SNP标记为中国荷斯坦奶牛产奶性状及体细胞评分的标记辅助选择提供了科学依据。
摘要（英）：

The invention relates to an SNP (Single Nucleotide Polymorphism) marker related with a Chinese Holstein cow milk production property and a somatic cell score and an application thereof. A site of the SNP marker is located on a Chinese Holstein cow HAL (Histidine Ammonia-lyase) gene, such as a 197bp site of a sequence shown as SEQ ID NO.1; a basic group of the site is G or A; the content of lactoprotein of the Chinese Holstein cow with the basic group G is obviously higher than that of the Chinese Holstein cow with the basic group A; and the somatic cell score of the former is obviously lower than the latter. The invention further provides a primer for detecting the SNP marker and a kit containing the primer. The invention further provides the application of the SNP marker to a Chinese Holstein cow advantageous variety with high identification lactoprotein content and low somatic cell score. The invention further provides an application of the SNP marker to assistant breeding in a Chinese Holstein cow molecular marker. The SNP marker disclosed by the invention provides scientific basis for marker auxiliary selection of the Chinese Holstein cow milk production property and the somatic cell score.

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