The invention discloses a fusion peptide with a myocardial preservation function as well as a preparation method and application thereof and belongs to the field of bio-pharmaceuticals. The fusion peptide with the myocardial preservation function is characterized by comprising an amino acid sequence shown in SEQ ID NO.1. The biological activity research on the obtained fusion peptide proves that the fusion peptide has the effect of treating or preventing arrhythmia, ventricular fibrillation, myocardial ischemia, myocardial infarction, myocardium cell apoptosis and other heart associated diseases. In addition, the invention also provides a method for preparing the fusion peptide and application of the fusion peptide in preparation of medicines with the myocardial preservation function.