The invention discloses a channel estimation method based on dynamic pilot frequency adjustment. The channel estimation method at least comprises the following steps of acquiring data frames which are currently input; subjecting the data frames to pilot frequency pattern analysis; performing channel estimation according to the result of pilot frequency pattern analysis to obtain mean square error (MSE) values; subjecting the MSE values of the data frames to MSE statistic analysis; judging whether the data frames need to be subjected to pilot frequency pattern adjustment; and using a pilot frequency pattern adjustment algorithm to confirm pilot frequency pattern adjustment strategies when the pilot frequency patterns need to be adjusted, and calling the pilot frequency patterns in a pilot frequency pattern base for adjustment. Compared with traditional fixation pilot frequency pattern channel estimation methods, the channel estimation method has higher estimation accuracy so that the whole transmission performances of communication systems in satellite moving multi-path channel environments are effectively improved.