The invention relates to a method for preparing duck hemorrhagic ovaritis inactivated vaccines, and belongs to the field of biotechnology. The method for preparing the duck hemorrhagic ovaritis inactivated vaccines includes the following steps: (1) culturing of cells used for vaccine preparation, (2) virus inoculation and culture, (3) virus liquid harvest, (4) virus liquid inactivation, and (5) vaccine preparation. Cells used for vaccine preparation are screened, matching degree between the cells and the virus is strengthened, a riptide perfusion type bioreactor culture system is used for improving multiplication titer and harvest yield of the virus, and a whole production process does not involve other biosafety and public health problems and is suitable for large scale production.