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发明公开
CN103160547A 一种醇脱氢酶在催化生成(R)-4-氯-3羟基丁酸乙酯中的应用 无效 - 撤回

一种醇脱氢酶在催化生成(R)-4-氯-3羟基丁酸乙酯中的应用
摘要：
本发明公开了一种氨基酸序列如SEQIDNO：2所示的醇脱氢酶在4-氯乙酰乙酸乙酯不对称还原制备(R)-4-氯-3羟基丁酸乙酯中的应用。即以氨基酸序列如SEQIDNO：2所示的醇脱氢酶为催化剂，以4-氯乙酰乙酸乙酯为底物，以NADH为辅因子，不对称还原制备(R)-4-氯-3羟基丁酸乙酯。本发明首次将氨基酸序列如SEQIDNO：2所示的醇脱氢酶应用于4-氯乙酰乙酸乙酯不对称还原制备(R)-4-氯-3羟基丁酸乙酯中，取得很好的效果，其酶活高达5.6U/mg，其对底物的得率高达94%、产物的对映体过量值为100％，且产量高，大大降低了生产成本。
摘要（英）：

The invention discloses application of alcohol dehydrogenase with amino acid sequence disclosed as SEQ ID NO:2 in preparing ethyl (R)-4-chloro-3-hydroxy butyrate from ethyl 4-chloroacetoacetate by asymmetric reduction. By using alcohol dehydrogenase with amino acid sequence disclosed as SEQ ID NO:2 as a catalyst, ethyl 4-chloroacetoacetate as a substrate and NADH (nicotinamide adenine dinucleotide) as a cofactor, asymmetric reduction is carried out to prepare the ethyl (R)-4-chloro-3-hydroxy butyrate. The invention applies the alcohol dehydrogenase with amino acid sequence disclosed as SEQ ID NO:2 in preparing ethyl (R)-4-chloro-3-hydroxy butyrate from ethyl 4-chloroacetoacetate by asymmetric reduction for the first time, and has favorable effect. The enzyme activity is up to 5.6 U/mg, the yield of the substrate is up to 94%, and the enantiomeric excess value of the product is 100%. The yield is high, and the production cost is greatly lowered.

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