The invention discloses seasonings for grilling fish and a preparation method of the seasonings. The raw materials of the seasonings for grilling fish comprise clear water, yeast extract, fish hydrolyzate, sugar, salt, monosodium glutamate, maltodextrin, cumin powder, garlic powder, star anise powder, chili powder, chili oil, modified starch, xanthan gum, meat flavor, grilled fish essential oil and potassium sorbate. The seasonings for grilling fish is prepared by the yeast extract compounding with spices through Maillard reaction and a blending technique, not only has the special grilled fish flavor, rich taste and long-lasting aftertaste, but also is rich in nutrition due to the yeast extract. In addition, when used for grilling fish, the seasonings can be directly added, so that the time for blending spices is reduced, the labor intensity for grilling is reduced, and the seasonings are convenient to use.