The embodiment of the invention provides an oxide thin film transistor array substrate, a manufacturing method of the array substrate, and a display panel. The leakage current of an oxide thin film transistor (TFT) can be reduced; the phenomena of picture flickering, crosstalk, residual image and the like of the display panel can be overcome to some extent; and the display performance can be improved. The oxide TFT array substrate comprises a substrate, an oxide TFT, a grid line, a data line and a pixel electrode, wherein the oxide TFT, the grid line, the data line and the pixel electrode are arranged on the substrate; a drain of the oxide TFT is connected with the pixel electrode; a guide structure is arranged between a source of the oxide TFT and the data line; the source is connected with the data line through the guide structure; and the resistivity of the guide structure is greater than that of source metal. The oxide thin film transistor array substrate disclosed by the embodiment of the invention is suitable for the technical field of display.