A large-span and heavy-load multi-layer open cut tunnel lining structure reduces concrete hydration heat generated in lining structure pouring greatly, ensures the concrete construction quality, and improves the integrity, the impermeability and the durability of the lining structure. The large-span and heavy-load multi-layer open cut tunnel lining structure comprises an inner layer of lining and at least one outer layer of lining; and the inner layer of lining and the outer layer of lining are in driving fit with each other, and are connected through a pre-cast connecting reinforcement to form an integral structure.