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发明公开
CN103243105A 一种旋毛虫重组蛋白及应用 有权

一种旋毛虫重组蛋白及应用
摘要：
本发明公开一种重组蛋白的制备方法，特别是一种TsSerpin重组蛋白，以及这种蛋白和它的用途。本发明的TsSerpin重组蛋白的制备方法是：以猪旋毛虫肌幼虫中提取总RNA为模板，以Oligo(dT)18Primer为引物进行反转录；再用上述所得到的反转录cDNA为模板，用SEQID№1和SEQID№2为引物进行PCR扩增，得到猪旋毛虫TsSerpin基因；再经构建重组质粒、转化大肠杆菌等处理得到目标蛋白。本发明的重组蛋白可用于制备检测旋毛虫病的抗原。
摘要（英）：

The invention discloses a preparation method of a recombinant protein and in particular relates to a TsSerpin recombinant protein and application thereof. The preparation method of the TsSerpin recombinant protein comprises the following steps: carrying out reverse transcription, with total RNA (Ribonucleic Acid) extracted from pig trichina muscle larva as a template and Oligo(dT)18Primer as a primer; with the obtained reverse transcription Cdna (Deoxyribose Nucleic Acid) as a template and SEQ ID No.1 and SEQ ID No.2 as primers, carrying out PCR (Polymerase Chain Reaction) amplification, to obtain pig trichina TsSerpin gene; and carrying out the treatment including recombinant plasmid construction, escherichia coli conversion and the like to obtain target protein. The recombinant protein disclosed by the invention can be used for preparing an antigen for detecting trichinosis.

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