The invention discloses a substrate cleaning device. The substrate cleaning device comprises a shell, a plurality of spraying pipelines installed on the shell in a pivoted mode and distributed side by side in the moving direction of a substrate, and a driving system, wherein a cleaning chamber is arranged in the shell, each spraying pipeline is provided with a plurality of liquid spraying openings guiding liquid in the spraying pipeline to a face to be cleaned of the substrate, and a transmission gear coaxially fixed with each spraying pipeline is arranged at one end of each spraying pipeline; the driving system drives the spraying pipelines to do same-frequency reciprocating rotation through the transmission gears, and the direction of the reciprocating rotation of a part of the spraying pipelines is opposite to the direction of the reciprocating rotation of the other part of the spraying pipelines. The substrate cleaning device can improve the cleaning effect on the surface of the substrate on the premise that influences on the moving speed of the substrate are small.