The invention relates to a processing method of acid-containing crude oil. The method includes: (1) mixing acid-containing crude oil with certain amount of water to conduct aquathermolysis to remove acid; and (2) subjecting the crude oil that undergoes aquathermolysis and acid removal to electric desalting and dewatering treatment, thus obtaining the acid, salt, calcium and iron ion removed crude oil. With the method provided in the invention, the acid removal rate of the aquathermolysis acid removal treatment of the acid-containing crude oil can reach over 85%. The naphthenate causing emulsification is converted into a water soluble salt, and then electric desalting and dewatering are carried out, so that the desalting and dewatering efficiency can be obviously enhanced. The content of the water soluble salt in the crude oil can be reduced to less than 3mg/l, more than 90% of calcium and iron metal ions can be removed, and simultaneously the water consumption in the desalting and dewatering process of the acid-containing crude oil can be reduced by over 50%.