The invention discloses a brucella abortus indirect enzyme linked immunosorbent assay (ELISA) detection kit which consists of the following reagents: (1) a pre-enveloped ELISA reaction plate comprising enveloping liquid, an enveloping antigen and closing liquid, (2) plumbous stearate (PBST) washing liquid, (3) IgG-HRP, (4) ending liquid, and (5) color developing liquid, wherein the enveloping antigen is a Virb8 protein. The Virb8 protein related by the invention can be only specifically expressed in the early stage of the brucella abortus infection; an antibody correspondingly produced by the protein is in the early stage of the infection; the produced antibody can live for a long time, so that whether an antibody titer caused by the brucella abortus infection and the quantitative infection exists can be specifically and sensitively judged by cloning and expressing the brucella abortus Virb8 protein and constructing a corresponding indirect ELISA detection method; a quick and accurate method can be supplied to early serologic diagnosis of the brucella abortus disease; the brucella abortus indirect ELISA detection kit has a great practical significance for a brucella abortus site detection technology for a large batch of samples.