The invention relates to a female backcross method for establishing silkworm sex-linked balanced lethal systems. Female moths of conventional varieties and male moths of a silkworm sex-linked balanced lethal system are crossed to obtain F1 generation. Male moths of the F1 generation and female moths of the silkworm sex-linked balanced lethal system are backcrossed to breed BC1 generation which is separated into egg ring I type and egg ring II type according to lethal time difference. After female moths of the egg ring II type mate with male moths of the egg ring I type, a silkworm sex-linked balanced lethal system H1 can be established. At the same time, female moths of the conventional varieties and male moths of the egg ring I type are backcrossed to obtain BC2 generation. The bred female moths of the silkworm sex-linked balanced lethal system H1 and male moths of the BC2 generation are crossed to breed egg ring I type and egg ring II type according to lethal time difference. After circulation according to the above steps, a silkworm sex-linked balanced lethal system Hn which meets target requirements can be established. The method has advantages of simple operation, fewer steps and high efficiency, is easy to establish and obtain a silkworm sex-linked balanced lethal system which basically maintains various characters of conventional varieties, and is suitable for popularization and application.