The invention provides a determination method of filter tip interception of ammonia in mainstream smoke, which is characterized in that: four smoked filter tips are longitudinally cut and put into a 250 mL conical flask, 100 mL of 10 mM hydrochloric acid is exactly added, oscillation and extraction last for 40 min, the solution is rested for 5 min, 2 mL of extract is filleted by 0.45 [mu]m membrane for purification, filtrate is analyzed by ion chromatography, qualitative analysis is performed by comparing retention time of chromatographic peaks of standard samples and filter tip samples, and quantification is realized by the external standard method. The determination method of filter tip interception of ammonia in mainstream smoke is good in repeatability and recovery, and is suitable for the analysis of large numbers of samples.