The invention discloses a silk ilk bracket as well as a preparation method and application thereof, and a three-phase silk ligament graft and a preparation method thereof. Firstly, silks are woven into a net-shaped bracket with a macroporous structure; then the net-shaped silk bracket is divided into three areas: A. a ligament area, B. a cartilage modification area and C. a bone modification area; ligament cells, chondrocyte and osteoblast are respectively planted in the areas A, B and C; the net-shaped bracket modified by the divided areas is rolled to form a cylindrical ligament graft with a physiological transition structure. The structure gradually transited from soft tissues to hard tissues effectively avoids the problem of stress concentration due to direct connection of the soft and hard tissues, solves the problem of weak biological fixation of the ligament graft-bone combination part in the existing single-phase tissue engineering ligament, and solves the problem that the seed cells cannot enter the deep part of the ligament in the traditional ligament weaving method; the ligament graft has the complete weaving structure, and solves the problem of weak connection between layers in the existing three-phase tissue engineering ligament.