The invention relates to a preparation method of chiral alkynyl tertiary alcohol and use thereof. The method comprises the following steps: mixing racemic hydroxy acetylenic acid and a solvent isopropyl alcohol with chiral pure S-(-)-methylbenzylamine or R-(+)-methylbenzylamine to split out chiral hydroxy acetylenic acid, and adding the chiral hydroxy acetylenic acid and a cuprous chloride catalyst into an acetonitrile solution to react to obtain the chiral alkynyl tertiary alcohol. The chiral alkynyl tertiary alcohol can not only be used for preparing natural chiral abscisic acid and enantiomers thereof, but also used for synthesizing chiral abscisic acid analogues. The method is low in cost, good in splitting effect and capable of being used in a large scale to overcome the defects of an existing chemical synthesis method of abscisic acid of expensive chiral auxiliary reagent, low yield and difficulty in realizing mass production.