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发明公开
CN103509877A 一种用于检测PRV的荧光定量PCR试剂盒及其应用 有权

一种用于检测PRV的荧光定量PCR试剂盒及其应用
摘要：
本发明涉及一种特异性检测猪伪狂犬病病毒（PRV）感染的荧光定量PCR快速检测试剂盒及其应用，所述试剂盒包括用于扩增猪伪狂犬病病毒gE基因保守区域的特异性引物对和特异性的TaqMan荧光标记探针，其中，所述特异性引物对的上游引物序列为5’-GAGGACGACGGGCTGTAC-3’，特异性引物对的下游引物序列为5’-GGACATCAACAGGCGGTTG-3’，所述TaqMan探针的序列为5’-TGGGTCCATTCGTCACTTCCG-3’，且TaqMan探针的5’端标记荧光报告基团FAM，3’端标记本身不发光的荧光淬灭基团BHQ1。本发明的试剂盒可用于快速定性定量检测PRV感染、鉴别检测PRV gE基因缺失疫苗毒株和/或PRV野毒株。
摘要（英）：

The invention relates to a fluorescence quantitative PCR rapid detection kit used for specifically detecting pig pseudorabies virus (PRV) infection, and an application thereof. The kit comprises a specific primer pair used for amplifying PRV gE gene conserved region, and a specific TaqMan fluorescent-labeled probe. An upstream primer sequence of the specific primer pair is 5'-GAGGACGACGGGCTGTAC-3', and a downstream primer sequence of the specific primer pair is 5'-GGACATCAACAGGCGGTTG-3'. The sequence of the TaqMan probe is 5'-TGGGTCCATTCGTCACTTCCG-3'. The 5' end of the TaqMan probe is labeled with a fluorescent reporter group FAM. The 3' end of the TaqMan probe is labeled with a fluorescence quenching group BHQ1 which is not intrinsically fluorescent. The kit can be used for rapid qualitative and quantitative detections of PRV infection, and can be used in identification detections of PRV gE gene-deleted vaccine strains and/or PRV wild strains.

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