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发明公开
CN103539842A 草鱼呼肠孤病毒Ⅱ型S10基因编码的重组蛋白、由其制备的多克隆抗体及应用 失效 - 权利终止 转让

草鱼呼肠孤病毒Ⅱ型S10基因编码的重组蛋白、由其制备的多克隆抗体及应用
摘要：
本发明公开了一种草鱼呼肠孤病毒Ⅱ型S10基因编码的重组蛋白、由其制备的多克隆抗体及应用。草鱼呼肠孤病毒Ⅱ型S10基因编码蛋白，其氨基酸序列如SEQIDNO:2所示，编码该蛋白的核苷酸序列如SEQIDNO:1所示。本发明获得的重组蛋白具有较好的免疫原性，与GCRV其它结构蛋白相比，其诱导免疫动物产生的特异性抗体效价更高。进一步试验表明，用S10重组蛋白免疫草鱼，可使草鱼诱导产生较高的特异性抗体，并能对草鱼呼肠孤病毒强毒株的攻击起到一定的免疫保护作用。因此，对GCRVⅡ型S10基因编码蛋白的研究和应用，对开发草鱼出血病新型疫苗及免疫学检测试剂盒具有极其重要的意义，为草鱼出血病提供新的有效解决途径。
摘要（英）：

The invention discloses a recombinant protein coded by a grass carp reovirus (GCRV) type-II S10 gene, a polyclonal antibody prepared from the recombinant protein and an application of the recombinant protein. The amino acid sequence of the GCRV type-II S10 gene-coded protein is shown by SEQ ID No:2, and the nucleotide sequence coding the protein is shown by SEQ ID No:1. The recombinant protein disclosed by the invention has good immunogenicity; compared with the proteins of other structures of GCRV, the specific antibody valence generated by inducing an immune animal is higher. Further tests indicate that by immunizing the grass carp with the S10 recombinant protein, the grass carp can be induced to generate high specific antibody, and certain immune protection effect can be realized against the attack of a virulent strain of GCRV. Therefore, the study and application of the GCRV type-II S10 gene-coded protein are of vitally important significance to the development of a novel vaccine for the hemorrhagic disease of grass carp and an immunological detection kit, and a new effective solution is provided for the hemorrhagic disease of grass carp.

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