The invention discloses a preparation method of zinc-rich barley seedling green beverage. Barley seedling juice extracting solution is extracted through implementing color protection, embedding, enzymolysis and separation for barley seedling powder; the zinc-rich barley seedling green beverage is prepared by mixing barley seedling beverage, homogenizing, disinfecting and canning. The barley seedling green beverage prepared by the invention has clean aroma, bright color and no bad flavor such as bitter taste and astringent taste, so that the drinking quality of the barley seedling beverage is improved; as the normal-temperature shelf life achieves 18 months, the chlorophyll preserving rate of the prepared barley seedling beverage achieves 62-70%.