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发明公开
CN103575886A 检测赭曲霉毒素A的酶联免疫试剂盒及其应用 有权

检测赭曲霉毒素A的酶联免疫试剂盒及其应用
摘要：
本发明提供了一种检测赭曲霉毒素A的酶联免疫试剂盒，它含有：包被有赭曲霉毒素A偶联抗原的酶标板，赭曲霉毒素A标准品溶液，浓缩酶结合物，酶结合物工作液，底物显色液，终止液。本发明还公开了一种应用上述酶联免疫试剂盒检测样本中赭曲霉毒素A的方法，它包括：首先进行样本前处理，然后用试剂盒进行检测，最后分析检测结果。本发明提供的酶联免疫试剂盒可用于检测饲料（原料、配合料和浓缩料）样本中赭曲霉毒素A的残留量，其操作简便、成本低、灵敏度高、能够现场监控且适合大量样本的筛查。
摘要（英）：

The invention provided an enzyme linked immunosorbent assay kit used for detecting ochratoxin A. The enzyme linked immunosorbent assay kit comprises an elisa plate containing ochratoxin A coupling antigen, a standard solution of ochratoxin A, a concentrated enzyme conjugate, an enzyme conjugate working solution, a substrate color development solution, and a stopping solution. The invention also discloses a method used for detecting ochratoxin A using the enzyme linked immunosorbent assay kit, and comprises steps of pretreatment of samples, detection via the enzyme linked immunosorbent assay kit, and analysis of detection results. The enzyme linked immunosorbent assay kit can be used for detecting ochratoxin A residual quantity in forage (raw materials, auxiliary materials and concentrates); operation is simple and convenient; cost is low; sensitivity is high; and the enzyme linked immunosorbent assay kit is capable of realizing on-site monitoring, and is suitable for screening of a large amount of samples.

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