The invention discloses a feed capable of promoting molting of procambarus clarkia and a preparation method of the feed and belongs to the technical field of aquatic feeds. The feed is prepared from fish meal, meat and bone meal, soya bean meal, rapeseed meal, peanut meal, wheat, bran, rice bran, inkfish cream, crushed shrimp head meal, monocalcium phosphate, crystal lysine, crystal methionine, an anthraquinone extract, 35% vitamin C phosphate, a bonding agent, ecdysone, table salt and a premix. The preparation method comprises the steps that the raw materials are agitated and uniformly mixed and then a mixture is crushed by an ultrafine grinder to obtain a mixed material; finally, the mixed material is prepared into pellet feeds by a conventional method of pellet feeds. The pellet compound feed has comprehensive and abundant nutrition; when the feed is applied to the cultivation of the procambarus clarkia, the immunity and the anti-stress capability of the procambarus clarkia are improved, the normal and synchronous molting of the procambarus clarkia is promoted, and the probability of autotomy is reduced; the palatability of the feed is improved; the healthy growth of the procambarus clarkia is promoted.