The present invention discloses a bacterial agent for preparing an organic fertilizer by adopting countryside organic waste as a fermentation raw material, and an application thereof, wherein the active components of the bacterial agent comprise Trichoderma viride and Saccharomyces cerevisiae. According to the present invention, the organic fertilizer prepared from the bacterial agent adopting the countryside organic waste as the fermentation raw material provides significant yield increase effects for cucumber and green pepper, wherein the yield of the cucumber can be increase by 21.98%, and the yield of the green pepper can be increase by 19.71%; and application of the organic fertilizer preparation bacterial agent adopting the countryside organic waste as the fermentation raw material to prepare the organic fertilizer is the effective approach for countryside organic waste resource utilization, and the fermentation process is simple and easy to perform, and is suitable for promotion and application in the countryside areas.