The invention discloses a construction method for a field plot trial of fertilizer application to paddy fields. Through the selection of a test field, ridges of plots and footpaths are constructed by repeatedly settling moist soil in a paddy field; a plot irrigation mode that main grooves are connected with small grooves is adopted so as to increase the repeated treatment times of each plot; the plots are set in a block so as to ensure that the density of transplants and the number of seedlings in the plots are identical. The construction method comprises the following steps: A, selecting the test field; B, setting the plots; C, constructing the plots; D, applying fertilizers to the plots; F, raising and transplanting seedlings; G, irrigating the plots; H, intertilling and weeding; I, controlling pest; J, harvesting rice; K, sampling soil and ground water. The test plots are simple in structure and low in construction cost, and the cross flow of water and nutrients among the plots are effectively avoided. Therefore, the construction method is suitable for various field plot trials of short-term and long-term fertilizer application, agricultural chemicals, varieties, irrigation, cultivation, discharge of greenhouse gasses, and runoff and leaching loss of nitrogen and phosphorus nutrients.