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发明公开
CN103834657A 一种人工DNA分子及表达目标基因的方法 失效 - 权利终止

一种人工DNA分子及表达目标基因的方法
摘要：
本发明公开了一种人工DNA分子及表达目标基因的方法。该人工DNA分子的碱基序列为SEQ？ID？NO.2，能够编码TALE-TF1，所述TALE-TF1能够识别SEQ？ID？NO.1中的28-48位；或所述人工DNA分子的碱基序列为SEQ？ID？NO.3，能够编码TALE-TF2，所述TALE-TF2能够识别SEQ？ID？NO.1中的42-62位；或所述人工DNA分子的碱基序列为SEQ？ID？NO.4，能够编码TALE-TF3，所述TALE-TF3能够识别SEQ？ID？NO.1中的56-76位。本发明的人工DNA分子能够激活绵羊成纤维细胞中β-酪蛋白基因启动子，为绵羊乳腺生物反应器的构建提供直接方便的检测技术。
摘要（英）：

The invention discloses an artificial DNA molecule and a target gene expression method. A base sequence of the artificial DNA molecule is shown in the formula of SEQ ID NO.2 and can code TALE-TF1 and the TALE-TF1 can recognize the sequence from 28-48 sites shown in the formula of SEQ ID NO.1; or the base sequence of the artificial DNA molecule is shown in the formula of SEQ ID NO.3 and can code TALE-TF2 and the TALE-TF2 can recognize the sequence from 42-62 sites shown in the formula of SEQ ID NO.1; or base sequence of the artificial DNA molecule is shown in the formula of SEQ ID NO.4 and can code TALE-TF3 and the TALE-TF3 can recognize the sequence from 56-76 sites shown in the formula of SEQ ID NO.1. The artificial DNA molecule can activate a beta-casein gene promoter of sheep fibroblasts and provide a direct and convenient detection technology for construction of a sheep mammary gland bioreactor.

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