The invention discloses a method for fresh-keeping and quarantining fruits. The method comprises the following steps of (a) boxing the fruits; (b) irradiating a fruit box by utilizing an electronic beam: the total irradiation dosage of the electronic beam is 0.3 to 2.0kGy, the total irradiation times of the electronic beam is 1 to 4 times, and the single irradiation dosage of the electronic beam is 0.3 to 0.6kGy; (c) decompressing and storing: decompressing and storing the fruit box after being irradiated by the electronic beam. The low-dosage multistage irradiation of the electronic beam is combined with the decompression process, the microorganism, insect pests and enzyme activity of the fruits can be effectively inhibited or killed, so that the refreshing time and shelf life of the fruit can be effectively prolonged, and the quarantining pesticide purpose can be realized; meanwhile, the original package of the fruit does not need to be damaged in real production, so that convenience in operation is realized, and the processing capacity is strong.