The invention relates to a high-protein tilapia fish ball and a preparation method thereof and belongs to the field of food processing technology. The invention relates to a fish ball, in particular relates to the high-protein tilapia fish ball and also a method for producing the high protein tilapia fish ball by utilizing a superhigh pressure technology. According to the preparation method, fish meat and fish skin of tilapia fish are used as main ingredients, soy protein, glutamine transaminase, konjac powder, sodium lactate, salt, granulated sugar, monosodium glutamate, white pepper powder and yellow wine are added, a superhigh pressure device with a temperature control device is utilized for processing the fish ball, pressure is controlled to be 200-600MPa, temperature is controlled to be 65-80 DEG C, and action time is controlled to be 10-20min to prepare the high-protein tilapia fish ball. The high-protein tilapia fish ball is good in flavor, high in protein content, compact in structure and good in elasticity, water-retaining property and anti-freezing effect.