The invention discloses a plum juice and straw mushroom seasoning and a production method thereof. The plum juice and straw mushroom seasoning is mainly composed of 40-65% by volume of a plum juice and 35-60% by volume of a straw mushroom extract liquid. The production method of the plum juice and straw mushroom seasoning comprises the following steps: pulping fresh straw mushroom, adding protease and cellulase for enzymatic hydrolysis, filtering, adding water to filter residues in order to carry out heat extraction, mixing the above obtained enzymatic hydrolysis liquid with the obtained heat extraction liquid, carrying out low temperature vacuum concentration, mixing the obtained concentrate with a preprocessed plum juice according to the above volume proportions, and carrying out precipitation and heating disinfection treatment to prepare the plum juice and straw mushroom seasoning. The production method has the advantages of low cost and environmental protection, and the plum juice and straw mushroom seasoning prepared by adopting the method has the fresh taste of the straw mushroom and also has the plum fragrance of the plum juice.