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发明公开
CN103911366A 一种基因组DNA提取方法及试剂盒 无效 - 驳回

一种基因组DNA提取方法及试剂盒
摘要：
本发明公开了一种基因组DNA提取方法及试剂盒，该试剂盒包含研磨器、溶液A（裂解液）、溶液B（DNA结合液）、溶液C（DNA洗涤液）、溶液D（DNA洗脱液）和纯化柱。该发明的试剂盒，成本低廉，试剂成分简单，操作流程少，不仅适用于微生物基因组的提取而且还适用于动物组织和植物组织的基因组的提取。同时提取基因组的过程耗时短（30min以内），提取的基因组质量高，基因组条带大小约为23kb，基因组的量可稳定保持在1μg-30μg的范围内；提取的过程不会用到有毒的试剂苯酚、氯仿，能很好的满足SouthernBlot，PCR，RFLP，DNA文库构建等各种分子生物学实验。
摘要（英）：

The invention discloses a genome DNA extraction method and a kit. The kit comprises a grinder, a solution A(lysate), a solution B(DNA combination liquid), a solution C(DNA washing liquid), a solution D(DNA eluate) and a purifying column. The provided kit has the advantages that cost is low, the reagent component is simple, the operation flow is little, and the kit is suitable for extraction of microbe genome and is also suitable for extraction of animal tissue genome and plant tissue genome. The time consuming of a process for extracting the genome is short (in 30 minutes), the quality of the extracted genome is high, the size of the genome strip is 23kb, the genome amount can be kept in a scope of 1mumg-30mumg in a stable mode; a toxic reagent phenol and chloroform are not used in the extraction process, and various molecular biology experiments for SouthernBlot, PCR, RFLP, DNA library construction can be better satisfied.

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