The invention provides application of vaccarin for resisting oxidation and high-glucose damage, and belongs to the technical field of traditional Chinese medicament application. The application first discovers that vaccarin has the activity of protecting oxidative stress injury and high-glucose damage of endothelial cells and can be used for treating a series of diseases induced by atherosclerosis, cataract, aged-related macular degeneration and other endogenous or exogenous active oxygen invasions, and preventing and treating diabetes mellitus, hypertension and related diseases caused by high glucose. The vaccarin can be applied to medicinal preparations, nourishment, healthy products, cosmetics and the like.