The invention discloses a preparation method of a seasoning with mixed chicken powder and seaweed flavors and belongs to the technical field of the food processing. The preparation method of the seasoning with the mixed chicken powder and seaweed flavors takes seaweed and chicken powder as main raw materials and comprises the following steps: selecting the raw material seaweed, immersing, cutting and pulping the seaweed, extracting with aid of microwave, carrying out enzymolysis and deodorization, filtering, adding the chicken powder, mixing, homogenizing, concentrating in vacuum, carrying out spray drying, collecting powder, and packaging, thus finally obtaining the product. The seasoning with the mixed chicken powder and seaweed flavors well maintains the seaweed flavor and the biological characteristics, has the chicken flavor characteristic, is good in brewing property, easily soluble in water, delicate in taste and rich in chicken flavor and seaweed flavor; the preparation method of the seasoning is simple, reasonable and applicable to industrialized production; the produced product is good in quality and long in quality guarantee period, thus facilitating distribution and sale.