The invention provides a soy isoflavone supported biological composite material porous scaffold and preparation. According to the porous scaffold, a nano calcium-deficient apatite-poly-amino acid copolymer composite material porous scaffold is used as a carrier, soy isoflavone is mixed in the carrier material, particularly, plant sourced 5,4',7-genistein is used as a growth factor component, wherein the mass ratio of the growth factor and the composite material is (1-10):100. When the porous scaffold is prepared, the components and a calcium sulphate dihydrate component foaming agent which is 5-15 percent by mass of the composite material are mixed, and are subjected to injection molding in an injection manner. Experiment results indicate that the cell proliferation degree of the porous scaffold to MG-63 is remarkably superior to that of a scaffold without supporting of soy isoflavone, the porous scaffold has significant difference and has an excellent prospect in serving as a scaffold material with excellent osteogenesis promoting performance.