The invention discloses a method for quickly detecting the content of insoluble water in a jet fuel. The method comprises the following steps: at different temperatures, mixing water with the jet fuel, violently shaking, subsequently standing, layering and taking supernatant liquid for testing, and establishing a calculation formula I which expresses the variation of the content of soluble water in the jet fuel along with the change of the environment temperature according to a testing result; testing the content of the soluble water in the jet fuel under different environment humidities in a determined temperature range, and establishing a calculation formula II which expresses the variation of the content of the soluble water in the jet fuel along with the change of the environment humidity under different environment humidities according to the testing result, detecting the total water content T in a jet fuel sample to be detected, and calculating the content R of the soluble water in the jet fuel sample to be detected according to the formulas I and II so as to obtain a content F of insoluble water in the jet fuel sample to be detected, wherein F is equal to T minus R. The method has the advantages of short detection time, accurate detection result and small error and is suitable for field fast detection of the content of water in the jet fuel.