The invention relates to the technical field of optical protection films, and especially relates to an optical protection film with 3D visual effect and a preparation method thereof. The preparation method comprises the following steps: selecting multifunctional acrylic polyurethane resin with the viscosity of 8000-20000 cps; using laser to carve a tooth on a lamellar scraper, wherein the tooth has a line-shaped structure formed by multiple semicircles; employing a template process to coat a surface of a PET transparent base material with multifunctional acrylic polyurethane resin by using the scraper; using a guide roller to send the PET transparent base material to a UV light box, so as to rapidly solidify multifunctional acrylic polyurethane resin under radiation of a UV light; and coating the other surface of the PET transparent base material with glue, performing baking solidification by a baker, and then pasting a PET film-protecting layer, so as to prepare the optical protection film with the 3D visual effect. The preparation method is simple, the optical protection film is good in 3D visual effect, the film is formed at a time, the production cost is low, and the preparation method is safe and environment-friendly.