The invention relates to a nanopore DNA (Deoxyribose Nucleic Acid) sequencing sensor for detecting DNA deoxyribonucleotide base sequence. The sensor comprises a current detection unit, a molybdenum disulfide field-effect tube, an atomic power microscope feeding system and an array unit, wherein DNA is bonded on an atomic power microscope probe by a chemical modification method; by virtue of a feeding control system of the atomic power microscope, a speed for driving DNA to go in and out of the nanopore is controlled to be 1nanometer per second; the speed fully meets the requirement of bandwidth for detecting DNA sequencing current signal; the nanopore is formed in a molybdenum disulfide nanoribbon in the process of penetrating through the pore of DNA; the molybdenum disulfide nanoribbon plays the role of the field-effect tube in the process of detecting the current signal and can be used for amplifying a signal for showing the DNA penetrating through the hole in real time and effectively increasing a signal to noise ratio; in addition, the method for arraying the atomic power microscope probe corresponding to the molybdenum disulfide field-effect tube can be used for carrying out multi-channel parallel real-time sequencing on the DNA to be detected parallel at the same time, so that the time cost is greatly reduced.