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发明公开
CN104101702A 一种四溴双酚A的间接竞争酶联免疫检测方法 有权

一种四溴双酚A的间接竞争酶联免疫检测方法
摘要：
一种四溴双酚A的间接竞争酶联免疫检测方法，包括1)用四溴双酚A合成人工抗原；2)制备四溴双酚A多克隆抗体：以四溴双酚A与BSA的偶联物为免疫抗原，免疫昆明大白鼠并利用现有的多克隆抗体制备与纯化技术获得四溴双酚A多克隆抗体；3)间接竞争ELISA检测：选择包被抗原浓度为10μg/mL，并用碳酸盐缓冲液稀释；洗板，加入缓冲液封闭，再次洗板；进行竞争，洗板，加二抗，洗板；进行显色反应；最后酶标仪测定450nm的吸光值(OD450)。本发明建立了四溴双酚A的间接竞争ELISA方法，为环境中四溴双酚A残留检测提供了一种快速高效的检测手段。由于该方法采用的是多克隆抗体，成本较低且准确性和稳定性较好。
摘要（英）：

The invention discloses an indirect competition ELISA method for tetrabromo bisphenol A. The assay method comprises the following methods: 1) synthesizing an artificial antigen through the tetrabromo bisphenol A; 2) preparing a tetrabromo bisphenol A polyclonal antibody: taking the conjugate of the tetrabromo bisphenol A and BSA as an immunizing antigen, immunizing Kunming white rats, and utilizing the conventional polyclonal antibody preparation and purification technologies to acquire the tetrabromo bisphenol A polyclonal antibody; 3) assaying the indirect competition ELISA: choosing an envelope antigen of which the concentration is 10 [mu]g/mL, and performing dilution with a carbonate buffer solution; washing an ELISA plate, adding the buffer solution to seal the ELISA plate and washing the ELISA plate again; carrying out competition, washing the ELISA plate, adding a second antibody and washing the ELISA plate again; performing a chromogenic reaction; finally, measuring with a microplate reader to acquire that a light absorption value is 450nm (OD 450). The invention constructs the indirect competition ELISA method for the tetrabromo bisphenol A, and provides a quick and efficient assay means for assaying the residual tetrabromo bisphenol A in atmosphere; as the method adopts the polyclonal antibody, the cost is lower and the stability is better.

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