The invention discloses a penicillin antibiotic appearance evaluation method which is as follows: determining appearance standards of a certain penicillin antibiotic by use of spectrophotometric method, and comparing the determined appearance standards with the absorbance of other batches of solutions of the penicillin antibiotic to evaluate wether the appearance of the penicillin antibiotic product of the to-be tested batch is qualified. A penicillin antibiotic appearance naked eye observation method is replaced by the penicillin antibiotic appearance evaluation method, the appearance standard is quantifiable, easy to control, objective and accurate, and does not change with time, place, personnel changes. The penicillin antibiotic appearance evaluation method has the advantages of low cost, high precision and high efficiency in the popularization and application in industrial production.