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发明授权
CN104141009B 早期膀胱癌的多靶标检测方法 有权 转让

早期膀胱癌的多靶标检测方法
摘要：
本发明早期膀胱癌的多靶标检测方法，属于生物检测技术领域。包括下述步骤：(1)、提取患者尿液中所含的DNA；(2)、对步骤(1)获得的DNA进行亚硫酸氢盐转化处理；(3)、通过甲基化特异性荧光定量PCR对EOMES、GDF15、NID2、PCDH17、POU4F2、TCF21、ZNF154这七个标志物基因进行检测，以ALU‑C4作为内参基因；(4)、以步骤(3)中的CT值来判定患者是否为早期膀胱癌。本发明具有以下优点：采用多个生物标志物基因联合检测，在每个标志物的特异性都比较高的前提下，使用多个标志物联用的方式来提高检测的敏感性和特异性；根据多个生物标志物基因设计了特异性引物。
摘要（英）：

The invention relates to a multi-target detecting method for the early-stage bladder cancer, and belongs to the technical field of biological detection. The multi-target detecting method comprises the following steps of: (1) extracting DNA contained in urea of a patient; (2) carrying out hydrosulphite conversion treatment onto the obtained DNA; (3) detecting seven marker genes including EOMES, GDF15, NID2, PCDH17, POU4F2, TCF21 and ZNF154 by virtue of methylation specific fluorescence quantitative PCR, wherein ALU-C4 is used as a reference gene; (4), judging whether the patient suffers from the early-stage bladder cancer or not according to a CT value in the step (3). The multi-target detecting method disclosed by the invention has the following advantages: a plurality of biomarker genes are adopted for joint detection, and a mode of combining a plurality of markers is applied to improve detecting sensitivity and specificity on the premise of higher specificity of each marker; specific primers are designed according to the plurality of biomarker genes.

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