The invention relates to the technical field of artificial breeding, in particular to a secondary debonding method for acipenser dabryanus. The method includes the steps of placing fertilized eggs in a container after the fertilized eggs of acipenser dabryanus are hatched to the gap-shaped blastopore period-wide neural plate period and are hatched for 46 hours to 54 hours at the temperature of 18 DEG C, adding talcum powder with the mass concentration of 8% to 12% or milk powder with the mass concentration of 13% to 17%, stirring the mixture in the same direction for 15 minutes to 30 minutes, and placing the fertilized eggs in a sturgeon hatching device directly or after washing the fertilized eggs to continue to be hatched. By means of the method, stability of water flow in the hatching process is ensured, man-made stimulation of the critical period of fertilized egg hatching is reduced, the probability of fertilized egg blocking is greatly lowered, the probability of blocky water molds is lowered, the loss ratio of live eggs is decreased, and the hatchability is improved.