The invention discloses a drug-resistant bacteria antagonizing composition and a preparation method thereof. The drug-resistant bacteria antagonizing composition consists of 62.67-75 percent of ceftazidime pentahydrate, 20.9-25 percent of tazobactam, 0-7.3 percent of solubilizer and 0-8.11 percent of stable protective agent, but not comprises 75 percent of ceftazidime pentahydrate and 25 percent of tazobactam. The composition disclosed by the invention has the characteristics of time-ordered administration and long-effect administration and is sensitive to most conventional drug-resistant bacteria; proved by 337 clinical tests, compared with the corresponding single drugs, the drug-resistant bacteria antagonizing composition has the advantages of remarkable curative effect and fewer adverse effects, such as the side effect for respiratory tract infection and urinary system infection, accords with the drug development concept of network pharmacology, and is an effective means for the drug-resistant bacteria antagonizing treatment.