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发明公开
CN104178496A 植物低磷应答调控单元及表达载体构建技术 无效 - 撤回

植物低磷应答调控单元及表达载体构建技术
摘要：
本发明描述了一种植物低磷应答调控单元PSS(PHR1-35S-SIZ1)表达载体pX6-PHR1-35S-SIZ1的构建技术。该表达载体的构建方法是，从拟南芥中克隆得到SIZ1基因，将35S启动子插入到SIZ1基因的上游，构建35S-SIZ1表达单元，将该表达单元插入植物表达载体pX6，构建载体pX6-35S-SIZ1，再将PHR1基因插入该载体中，得到表达载体pX6-PHR1-35S-SIZ1。用表达载体pX6-PHR1-35S-SIZ1转化农杆菌GV3101，得到同时含有PHR1基因和SIZ1基因的工程农杆菌菌株NK-314。
摘要（英）：

The invention describes a construction technology of an expression vector pX6-PHR1-35S-SIZ1 of a plant low-phosphorus response regulation and control unit PSS (PHR1-35S-SIZ1). The construction method of the expression carrier comprises: cloning and obtain SIZ1 gene from arabidopis thaliana, inserting 35S promoter into the upstream of SIZ1 gene, so as to construct a 35S-SIZ1 expression unit, inserting the expression unit into a plant expression vector pX6 to construct a vector pX6-35S-SIZ1, then inserting PHR1 gene into the vector, so as to obtain pX6-PHR1-35S-SIZ1. The expression vector pX6-PHR1-35S-SIZ1 is used to transform agrobacterium GV3101 so as to obtain an engineering agrobacterium strain NK-314 containing both PHR1 gene and SIZ1 gene.

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