The invention discloses a forming and positioning device for an organ material of a flat-bottom bag. The forming and positioning device sequentially comprises a feeding device, a forming device, a shaping device, a second traction device, a servo conveying device, a pulling device, a cut-off device and a heat seal device, wherein the feeding device is used for providing an organ material thin film for the follow-up devices; the forming device is used for folding the two sides of the organ material thin film towards the centerline of the thin film, and the edges on the two sides are located on the centerline of the thin film after being folded; the shaping device is used for fixing the folded organ material thin film; the second traction device is used for providing power for forward movement of the organ material thin film; the servo conveying device is used for driving the organ material thin film to move forward; the pulling device is used for assisting in driving the organ material thin film to a set position; the cut-off device is used for cutting off the folded organ material thin film according to a set length and conveying the organ material thin film to a set position; the heat seal device is used for conducting heat seal positioning on the organ material and a main film of the flat-bottom bag. The forming and positioning device for the organ material of the flat-bottom bag is high in automation degree, stable, reliable and capable of promoting bag making efficiency.