The invention discloses a turnover control device on plunger type turnover and accumulation conveying equipment. The turnover control device is characterized in that a plunger hole is formed in the lower portion of each tray trolley, a turnover driving wheel disc is arranged at the shaft end of a transmission shaft at the end of a track lathe bed, a plunger is arranged on the outer edge of the turnover driving wheel disc and can be inserted into the corresponding plunger hole, and the turnover driving wheel disc is driven by the transmission shaft to rotate around the axis together; an air cylinder is arranged on the track lathe bed and located at the accumulation positions of the tray trolleys, and accumulation of the tray trolleys operating on the track lathe bed is controlled by a piston rod capable of ascending or descending in the air cylinder; a sensor signal triggering support synchronously rotating along with the transmission shaft is installed at the shaft end of the transmission shaft, a sensor is located at the position where the sensor can be triggered by the sensor signal triggering support, and the senor and the air cylinder are connected through a signal line and a PLC. The sensor is used for controlling the releasing time of the tray trolleys, and alignment between the plunger and the plunger holes in the turnover process is accurate and reliable.