The invention relates to a fluorescence labeling method for pyraoxystrobin and applications thereof. The invention discloses a rapid and efficient fluorescence labeling method for a pesticide pyraoxystrobin, which comprises simple steps, namely, under the action of a catalyst, pyraoxystrobin is amidated by using ethylenediamine, then obtained pyraoxystrobin derivatives and dansyl chloride are further amidated under the support of silver hydroxide to introduce a fluorescent group; the dansyl chloride labeled pyraoxystrobin has obvious fluorescence characteristic; by using a fluorescence confocal microscopy technology, parts by which the fluorescence labeled pyraoxystrobin passes in a living body can be determined, and then the metabolic pathway of the pyraoxystrobin can be traced and determined by the dansyl chloride fluorescence labeled pyraoxystrobin.