The invention relates to the field of food processing, and in particular relates to instant noodle seasoning powder and a preparation method thereof. The instant noodle seasoning powder is prepared from the following raw materials by the following steps according to certain proportions: a flavored morchella material, fresh beef, soybean paste, sesame oil, fresh onion, minced garlic, spice, light soy sauce, white sugar and table salt. The instant noodle seasoning powder disclosed by the invention not only reserves the fresh taste of morchella, but also has the flavors of other raw materials; under a circumstance of not adding monosodium glutamate, the prepared seasoning powder is intense in flavor, unique in taste, natural, healthy and nutrient.