The invention discloses a toothbrush flexible and automatic production line. The toothbrush flexible and automatic production line comprises an injection molding unit, a glue injection unit, a bristle planting unit, a bristle sanding unit, a cleaning and disinfection detecting unit and a packaging unit, materials are orderly transferred among the units through logistics equipment, the logistics equipment comprises a sorting and conveying manipulator between the injection molding unit and the glue injection unit, isometric conveying mechanisms among the subsequent units; by means of a modular structure, assembling can be conducted in accordance with production needs, each unit is provided with a corresponding sorting and conveying manipulator for independent operation, and connectors of the isometric conveying mechanism in the sorting and conveying manipulator and the independent isometric conveying mechanism are the same to guarantee connectors exchange; PLC (Programmable Logic Controller) networks based on the field bus are utilized in the production line, cooperative control is achieved among units, and video monitoring equipment is provided for effective control and condition monitoring of the production line. The toothbrush flexible and automatic production line has the advantages of realizing flexibility and automation of the production line, being capable of effectively improving toothbrush production efficiency and production quality, and greatly reducing labor intensity of workers and production cost.