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发明公开
CN104293721A 一种高密度培养枯草芽孢杆菌的方法 无效 - 驳回

一种高密度培养枯草芽孢杆菌的方法
摘要：
本发明涉及一种高密度培养枯草芽孢杆菌的方法，包括步骤：(1)以枯草芽孢杆菌为发酵菌种进行三级种子活化，其中，活化培养基的组份包括：蛋白胨10g/L、牛肉粉3g/L、氯化钠5g/L、葡萄糖1g/L；(2)将活化后的菌种接种到发酵培养基中培养，发酵培养基的组份包括：葡萄糖 10g/L、豆饼粉 15g/L、MnSO4 0.3g/L、NaCl 5g/L、K2HPO4 0.3g/L、碳酸钙5g/L；在30℃，搅拌转速200r/min条件下发酵48h后得高密度培养的枯草芽孢杆菌。本发明通过对培养基配方及发酵条件的改进，枯草芽孢杆菌的活菌数达到8.9×109cfu/mL以上，为提高枯草芽孢杆菌的工业化发酵提供了必要条件，可大大提高生产率。
摘要（英）：

The invention relates to a method for high-density culture of bacillus subtilis. The method comprises the steps of (1) carrying out three-level seed activation by regarding bacillus subtilis as fermentation bacteria, wherein a activation culture medium comprises the components: 10g/L of peptone, 3g/L of beef powder, 5g/L of sodium chloride and 1g/L of glucose; (2) inoculating activated bacteria to a fermentation culture medium for culture, wherein the fermentation culture medium comprises the components: 10g/L of glucose, 15g/L of soybean cake powder, 0.3g/L of MnSO4, 5g/L of NaCl, 0.3g/L of K2HPO4, 5g/L of calcium carbonate; fermenting the components at a temperature of 30 DEG C under a stirring rotating speed of 200r/min for 48h to obtain high-density cultured bacillus subtilis. By improvement of culture medium prescriptions and fermentation conditions, viable count of bacillus subtilis is more than 8.9*10<9>cfu/mL, necessary conditions are provided for industrial fermentation of bacillus subtilis, and rate of production can be increased greatly.

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