The invention belongs to the field of microorganisms, and particularly relates to a gossypol degradation strain coming from ruminant rumens and application thereof. The gossypol degradation strain is collected in a common microorganism center of the microorganism culture preservation and management committee of Chinese on August 23, 2013; and the culture preservation number is CGMCC No.8069. According to the gossypol degradation strain coming from ruminant rumens and the application, gossypol serves as a unique carbon source, the new strain with higher degradation activity to the gossypol is separated, screened, purified and cultivated from the ruminant rumens; through morphology and molecular biological identification, the strain RBS is Bacillus subtilis; and the homology between the Bacillus subtilis and the Bacillus tequilensis strain KM34 is 98%. The strain comes from the ruminant rumens of a living body, the nondeterminacy of the microorganism toxicology coming from soil or other natural environments is avoided, and a mice toxicity test shows that bacteria do not have toxicity. The liquid state fermentative gossypol degradation rate reaches 94.74%; after the bacteria are used for fermenting cottonseed meal, it is determined that the degradation rate of the free gossypol in the fermented cottonseed meal approaches to 80% through liquid chromatography (HPLC).