The invention discloses a method for preparing a negative electrode material of a titanium substrate/titanium spherical composite lithium ion battery by a one-step method. The method comprises the following steps: dissolving 0.5-3 mass parts of hexadecylamine into 100 volume parts of ethyl alcohol, adding 0.5-2 volume parts of strong ammonia water and 1-3 mass parts of titanium isopropoxide, adding 50 volume parts of distilled water containing 0.03-1 mass part of LiOH.H2O, moving a reaction liquid into a reaction kettle, reacting at 100-300 DEG C for 1-48 hours, cooling the kettle to room temperature, centrifuging and washing so as to obtain white precipitates, and drying the white sample; and forging the dried sample at 500-800 DEG C for 2 hours. The titanium substrate/titanium spherical composite material is synthesized successfully, and is applied to the negative electrode of a lithium ion battery, is charged and discharged for 500 times with the current density of 3500mA/g, and after cycling, the capacity is still above 100mAh/g.