The invention discloses a preparation method of a Maillard reaction flavor for cigarettes with medicinal herb fragrance characteristics, belonging to the technical field of cigarette flavors. The preparation method is performed according to the following steps: taking the following raw materials in parts by weight: 60 parts of natural herbal extracting liquid, 100 parts of 1,2-propylene glycol, 25 parts of xylose and 12.5 parts of an amino acid donor, adding into a reaction kettle, uniformly mixing, and reacting for 1-6 hours to obtain the Maillard reaction flavor for the cigarettes with the medicinal herb fragrance characteristics. According to the Maillard reaction flavor for the cigarettes with medicinal herb fragrance, prepared by using the method disclosed by the invention, a medicinal herb fragrance style of the flavor can be shown, fragrance types can be enriched, the smoke irritation can also be reduced, and the smoking taste of the cigarettes can be coordinated. The preparation method is simple to operate and easily-available in reaction substrates, and the prepared Maillard reaction flavor is stable in quality and significant in medicine fragrance style, and is harmonious with the smoking taste of the cigarettes.