The invention discloses a beta-cyclodextrin-modified gold nanoparticle/silica gel chiral stationary phase and a preparation method thereof. The stationary phase is prepared by the following steps: synthesizing a beta-cyclodextrin derivative with a sulfhydryl group, forming a gold nanoparticle shell on the surface of silica gel by using a sol-gel method, and connecting the beta-cyclodextrin derivative with the sulfhydryl group to the gold nanoparticle surface through an S-H key to form the chiral stationary phase, wherein the silica gel serves as a core, and the beta-cyclodextrin bonded gold nanoparticles are taken as the shell. The process is simple, the prepared separation material is novel in structure, the separation efficiency is high due to the gold nanoparticles, and the method is applied to separation of a chiral compound.